2022

(1) 原著論文 (Original Papers)

1. Nomura, R., Tsuzuki, S., Kojima, T., Nagasawa, M.,Sato, Y., Uefune, M., Baba, Y. Hayashi, T., Nakano, H., Kato, M., and Shimizu, M. (2022) Administration of Aspergillus oryzae suppresses DSS-induced colitis. Food Chem: Mol. Sci. 4, 100063. [Science Direct]

2. Uchida, S., Kojima, T., and Sugino, T. (2022) Frequency and Clinicopathological Characteristics of Patients With KRAS/BRAF Double-Mutant Colorectal Cancer: An In Silico StudyFrequency and Clinicopathological Characteristics of Patients With KRAS/BRAF Double-Mutant Colorectal Cancer: An In Silico Study. Pathol. Oncol. Res. 28, 1610206.[Pathol. Oncol. Res.]

3. Ashida, H., Murakami, K., Inagaki, K., Sawa, Y., Hemmi, H., Iwasaki, Y., and Yoshimura, T. (2022) Evolution and properties of alanine racemase from Synechocystis sp. PCC6803. J. Biochem: 171(4) 421-428. [Oxford Academic]

4. Wang, Y., Menon, A.K., Maki, Y., Liu, YS., Iwasaki, Y., Fujita, M., Guerrero, P.A., Silva, D.V., Seeberger, P.H., Murakami, Y., and Kinoshita, T. (2022). Genome-wide CRISPR screen reveals CLPTM1L as a lipid scramblase required for efficient glycosylphosphatidylinositol biosynthesis. Proc. Nat. Acad Sci USA: 119(14):e2115083119 [Europe PMC]

5. Damnjanović, J., Odake, N., Fan, J., Camagna, M., Jia, B., Kojima, T., Nemoto, N., Hitomi, K., and Nakano, H. (2022) Comprehensive analysis of transglutaminase substrate preference by cDNA display coupled with next-generation sequencing and bioinformatics. Sci. Rep. 12 13578. [Nature Scientific Reports]

6. Ojima-Kato, T., Nishikawa, Y., Furukawa, Y., Kojima, T., and Nakano, H. (2022) Nascent MSKIK peptide prevents or releases translation arrest in Escherichia coli. bioRxiv, preprint.［bioRxiv］

 

2021

(1) 原著論文 (Original Papers)

1. Hasegawa, T., Lucob-Agustin, N., Yasufuku, K., Kojima, T., Nishiuchi, S., Ogawa, A., Takahashi-Nosaka, M., Kano-Nakata, M., Inari-Ikeda, M., Sato, M., Tsuji, H., Wainaina, M. C., Yamauchi, A., and Inukai, Y. (2021) Mutation of OUR1/OsbZIP1, which encodes a member of the basic leucine zipper transcription factor family, promotes root development in rice through repressing auxin signaling. Plant Sci. 306, 110861. [Science Direct]

2. Sun, S., Araki, Y., Hanzawa, F.,Umeki, M., Kojima, T., Nishimura, N., Ikeda, S., Mochizuki, S., and Oda, H. (2021) High sucrose diet-induced dysbiosis of gut microbiota promotes fatty liver and hyperlipidemia in rats. J. Nutr. Biochem. 93, 108621. [Science Direct]

3. Uchida, S., Kojima, T., and Sugino, T. (2021) Clinicopathological Features, Tumor Mutational Burden, and Tumour-Infiltrating Lymphocyte Interplay in ERBB2-Mutated Breast Cancer: In Silico Analysis. Pathol. Oncol. Res. 27, 633243. [Pathol. Oncol. Res.]

4. Samantha, A., Damnjanović, J., Iwasaki, Y., Nakano, H., and Vrielink, A. (2021) Structures of an engineered phospholipase D with specificity for secondary alcohol transphosphatidylation: Insights into plasticity of substrate binding and activation. Biochem. J. 478, 1749-1767. [Portland Press] [Selected for Cover]

5. Ishikawa, M., Kojima, T., and Hori, K. (2021) Development of a Biocontained Toluene-Degrading Bacterium for Environmental Protection. Microbiol Spectr. 9, e0025921. [PubMed]

6. Wang, Y., Menon, A. K., Maki, Y., Liu, Y. S., Iwasaki, Y., Fujita, M., Guerrero, P. A., Silva, D. V., Seeberger, P. H., Murakami, Y., and Kinoshita, T. (2021) CLPTM1L is a lipid scramblase involved in glycosylphosphatidylinositol biosynthesis. [bioRxiv preprint.]

7. Oka, H., Kojima, T., Kato, R., Ihara, K., and Nakano, H. (2021) Construction of transcript regulation mechanism prediction models based on binding motif environment of transcription factor AoXlnR in Aspergillus oryzae. [bioRxiv preprint.]

8. Damnjanović, J., Odake, N., Fan, J., Jia, B., Kojima, T., Nemoto, N., Hitomi, K., and Nakano, H. (2021) cDNA display coupled with next-generation sequencing for rapid activity-based screening: Comprehensive analysis of transglutaminase substrate preference.[bioRxiv preprint.]

9. Kawai, T., Shibata, K., Akahoshi, R., Nishiuchi, S., Takahashi, H., Nakazono, M., Kojima, T., Nosaka-Takahashi, M., Sato, Y., Toyoda, A., Lucob-Agustin, N., Kano-Nakata, M., Suralta, R. R., Niones, J. M., Chen, Y., Siddique, K. H. M., Yamauchi, A., and Inukai, Y. (2021) WUSCHEL-related homeobox family genes in rice control lateral root primordium size. Proc Natl Acad Sci U S A. 119, e2101846119. [PNAS]

(2) 総説 (Reviews)

1. 加藤　晃代、中野　秀雄、兒島　孝明、永井　里美 (2021) 無細胞タンパク質合成系を利用した迅速抗体スクリーニング技術開発とその実用化. 生物工学会誌, 99, 62-67.　

 

2020

(1) 原著論文 (Original Papers)

1. Sila-on, D., Chertchinnapa, P., Shinkai,Y., Kojima, T., and Nakano, H. (2020) Development of a Dual Monoclonal Antibody Sandwich Enzyme-linked Immunosorbent Assay for the Detection of Swine Influenza Virus using Rabbit Monoclonal Antibody by Ecobody Technology. J. Biosci. Bioeng. 130, 217-225. [Science Direct]

2. Iwasaki, Y., Sakurai, Y., and Damnjanović, J. (2020) A simple chemo-enzymatic synthesis of alkyl-acyl (plasmanyl) phospholipids. Biocatal. Agric. Biotechnol. 26, 101625. [Science Direct]

3. Zhu, B., Gan, R., Cabezas, M. D., Kojima, T. Nicol, R., Jewett, M. C., and Nakano, H. (2020) Increasing cell‐free gene expression yields from linear templates in Escherichia coli and Vibrio natriegens extracts by using DNA‐binding proteins. Biotechnol. Bioeng. 117, 3849-3857. [Science Direct]

(2) 総説 (Reviews)

1. 兒島 孝明、岡 大椰、中野 秀雄 (2020) データマイニングによる転写因子の結合配列環境と発現応答の関連性の解析. 化学と生物, 58, 267-268.　

2. 岡 大椰、兒島 孝明、中野 秀雄 (2020) Aspergillus 属糸状菌由来転写因子を介した転写制御機構解析システム. 日本醸造協会誌, 115, 306-312.

3. Kojima, T. (2020) Ultra-high-throughput analysis of functional biomolecules using in vitro selection and bioinformatics. Biosci. Biotechnol. Biochem. 84, 1767-1774. [Taylor & Francis Online]

4. 岩崎雄吾、 Jasmina Damnjanović（2020) 改変型ホスホリパーゼDによる難合成性リン脂質の合成. 生物工学 98, 9, 481-484.

 

2019

(1) 原著論文 (Original Papers)

1. Oka, H., Kojima, T., Ihara, K., Kobayashi, T., and Nakano, H. (2019) Comprehensive investigation of the gene expression system regulated by an Aspergillus oryzae transcription factor XlnR using integrated mining of gSELEX-Seq and microarray data. BMC Genomics 20, 16. [PubMed]

2. Yamamoto N., Kashiwagi M., Ishihara M., Kojima T., Maturana A.D., Kuroda S., Niimi T. (2019) Robo2 contains a cryptic binding site for neural EGFL-like (NELL) protein 1/2. J. Biol. Chem. 294, 4693-4703. [J Biol Chem]

3. Alfi, A., Zhu, B., Damnjanović, J., Kojima, T., Iwasaki, Y., and Nakano, H. (2019) Production of active Manganese peroxidase in Escherichia coli by co-expression of chaperones and in vitro maturation by ATP-dependent chaperone release. J. Biosci. Bioeng. 128, 290-295. [Science Direct]

4. Kojima, T., Nakane, A. Zhu, B., Alfi, A., and Nakano, H. A simple, real-time assay of horseradish peroxidase using biolayer interferometry. Biosci. Biotechnol. Biochem. 83, 1822-1828. [Taylor & Francis Online]

5. Damnjanović, J., Nakano, H., and Iwasaki, Y. (2019) Acyl chain that matters: Introducing sn -2 acyl chain preference to a phospholipase D by protein engineering. Protein Eng. Des. Sel. 32, 1-11. [Oxford University Press]

6. Ohtsuka, H., Kato, T., Sato, T., Shimasaki, T., Kojima, T., and Aiba, H. Leucine depletion extends the lifespans of leucine-auxotrophic fission yeast by inducing Ecl1 family genes via the transcription factor Fil1. Mol. Genet. Genomics. 294, 1499–1509. [Springer Link]

(2) 総説 (Reviews)

1. Jasmina Damnjanović、岩崎 雄吾 (2019) 生物工学分野における分子ドッキングシュミレーションの応用　生物工学　97, 257-260.

2. 兒島 孝明、岡 大椰、中野 秀雄 (2019) バイオインフォマティクス技術を駆使した糸状菌トランスクリプトーム解析　生物工学　97, 265-268.

 

2018

(1) 原著論文 (Original Papers)

1. Mori, A., Ojima-Kato, T., Kojima, T., and Nakano, H. (2018) Zipbodyzyme: Development of new antibody-enzyme fusion proteins. J. Biosci. Bioeng. 125, 637-643. [Science Direct]

2. Damnjanović, J., Matsunaga, N., Adachi, M., Nakano, H, and Iwasaki, Y. (2018) Facile enzymatic synthesis of phosphatidylthreonine using an engineered phospholipase D. Eur. J. Lipid Sci. Technol. 120, 1800089 [Wiley]  [ Selected for Cover]

3. Panwad, R., Ojima-Kato, T., Damnjanović, J., Kojima, T., and Nakano, H. (2018) SKIK-zipbody-alkaline phosphatase, a novel antibody fusion protein expressed in Escherichia coli cytoplasm. J. Biosci. Bioeng. 126, 705-709. [Science Direct]

4. Kojima, T., Hata, J., Oka, H., Hayashi, K., Hitomi, K., and Nakano, H. (2018) Spatial arrangement of proteins using scCro-tag: application for an in situ enzymatic microbead assay. Biosci. Biotechnol. Biochem. 82, 1911-1921. [Taylor & Francis Online]

5. Wang, L., Iwasaki, Y., Andra, K.K., Pandey, K., Menon, A.K. and Bütikofer, P.: Scrambling of natural and fluorescently tagged phosphatidylinositol by reconstituted G protein-coupled receptor and TMEM16 scramblases. J. Biol. Chem. 293, 18318-18372 (2018). [JBC online]

6. Ojima-Kato, T., Morishita, S., Uchida, Y., Nagai, S., Kojima, T., and Nakano, H. (2018) Rapid Generation of Monoclonal Antibodies from Single B cells by Ecobody technology. Antibodies 7, 38. [MDPI]

(2) 総説 (Reviews)

1. Zinna, R., Gotoh, H., Kojima, T., and Niimi,T. (2018) Recent advances in understanding the mechanisms of sexually dimorphic plasticity: Insights from beetle weapons and future directions. Curr. Opin. Insect. Sci. 25, 35-41. [Science Direct]

2. Damnjanović, J., and Iwasaki, Y. (2018) Chapter 4: Enzymatic Modification of Phospholipids by Phospholipase D. in "Lipid Modification by Enzymes and Engineered Microbes" (U.T. Bornscheuer, ed.) , AOCS press, pp 69-88. [Science Direct]

3. Damnjanović, J., and Iwasaki, Y. (2018) Chap. 3: Enzymatic modification of phospholipids. in "Recent Advances in Biotechnology Vol. 4. Progress in Food Biotechnology" (Ali Osman, ed.). Bentham Science, pp.82-131. [Bentham Science]

 

2017

(1) 原著論文 (Original Papers)

1. Yabutani, T., Shimizu, S., and Nakano, H. (2017) Pilot-scale whole-cell biocatalysis for the hydroxylation of cyclosporine derivative, FR901459, at higher concentrations by Lentzea sp. 7887 using soybean flour as a novel substrate dispersant. J. Biosci. Bioeng. 123, 56-62. [PubMed]

2. Ojima-Kato, T., Nagai, S., and Nakano, H. (2017) N-terminal SKIK peptide tag markedly improves expression of difficult-to-express proteins in Escherichia coli and Saccharomyces cerevisiae. J. Biosci. Bioeng. 123, 540-546. [PubMed]

3. Yabutani, T., Tsujimoto, M., Ohira, S., Shimizu. S., and Nakano, H. (2017) Strain improvement of Lentzea sp. 7887 for higher yield per unit volume on hydroxylation of cyclosporine derivative FR901459. Biosci. Biotechnol. Biochem. 81, 1456-1459. [PubMed]

4. Mori, A., Ojima-Kato, T., Fuchi, S., Kaiya, S., Kojima, T., and Nakano, H. (2017) Development of a rapid immunoassay system: Luminescent detection of antigen-associated antibody-luciferase in the presence of a dye that absorbs light from free antibody-luciferase. J. Biosci. Bioeng. 124, 694-699. [PubMed]

5. Ojima-Kato, T., Yamamoto, N., Nagai, S., Shima, K., Akiyama, Y., Ota, J., and Tamura, H. (2017) Application of proteotyping Strain Solution ver. 2 software and theoretically calculated mass database in MALDI-TOF MS typing of Salmonella serotype. Appl. Microbiol. Biotechnol. 101, 8557-8569. [PubMed]

6. Ojima-Kato, T., Nagai, S., and Nakano, H. (2017) Ecobody technology: rapid monoclonal antibody screening method from single B cells using cell-free protein synthesis for antigen-binding fragment formation. Sci. Rep. 7, 13979. [nature.com]

(2) 総説 (Reviews)

1. 加藤 晃代、中野 秀雄 (2017) モノクローナル抗体取得技術の新展開 より良い抗体をより早く　化学と生物　55, 490-495.

2. 河合 翼、兒島 孝明、犬飼 義明 (2017) イネ根系に見られる表現型可塑性の分子機構解明を目指して　アグリバイオ　1(10), 47-50.

 

2016

(1) 原著論文 (Original Papers)

1. Damnjanovic, J., Kuroiwa, C., Tanaka, H., Ishida, K., Nakano, H., and Iwasaki, Y. (2016) Directing positional specificity in enzymatic synthesis of bioactive 1-phosphatidylinositol by protein engineering of a phospholipase D. Biotechnol. Bioeng. 113, 62-71. [Wiley]

2. Kojima, T., Mizoguchi, T., Ota, E., Hata, J., Homma, K., Zhu, B., Hitomi, K., and Nakano, H. (2016) Immobilization of proteins onto microbeads using a DNA binding tag for enzymatic assays. J. Biosci. Bioeng. 121, 147-153. [Science Direct]

3. Ujiie, A., Nakano, H., and Iwasaki, Y. (2016) Extracellular production of Pseudozyma (Candida) antarctica lipase B with genuine primary sequence in recombinant Escherichia coli. J. Biosci. Bioeng. 121, 303-309. [Science Direct]

4. Murzabaev, M., Kojima, T., Mizoguchi, T., Kobayashi, I., DeKosky, B. J., Georgiou, G., and Nakano, H. (2016) Handmade microfluidic device for biochemical applications in emulsion. J. Biosci. Bioeng. 121, 471-476. [Science Direct]

5. Omoto, E., Iwasaki, Y., Miyake, H., and Taniguchi, M. (2016) Salinity induces membrane structure and lipid changes in maize mesophyll and bundle sheath chloroplasts. Physiol. Plant. 157, 13-23. [Wiley]

6. Ojima-Kato, T., Fukui, K., Yamamoto, H., Miyake, S., Hirakawa, Y., Yamasaki, T., Kojima, T., and Nakano, H. (2016) "Zipbody" leucine zipper-fused Fab in E. coli in vitro and in vivo expression systems. Protein Eng. Des. Sel. 29, 149-157. [Oxford journals]

7. Muraki, M., Damnjanovic, J., Nakano, H., and Iwasaki, Y. (2016) Salt-induced increase in the yield of enzymatically synthesized phosphatidylinositol and the underlying mechanism. J. Biosci. Bioeng. 122, 276-282. [Science Direct]

8. Ojima-Kato, T., Yamamoto, N., Takahashi, H., and Tamura, H. (2016) Matrix-assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) Can Precisely Discriminate the Lineages of Listeria monocytogenes and Species of Listeria. PLoS One 11 e0159730. [PubMed]

9. Kojima, T., Kunitake, E., Ihara, K., Kobayashi, T., and Nakano, H. (2016) A Robust Analytical Pipeline for Genome-Wide Identification of the Genes Regulated by a Transcription Factor: Combinatorial Analysis Performed Using gSELEX-Seq and RNA-Seq. PLoS One 11 e0159011. [PubMed]

10. Inoue, A., Adachi, M., Damnjanovic, J., Nakano, H., and Iwasaki, Y. (2016) Direct enzymatic synthesis of 1-phosphatidyl-β-D-glucose by engineered phospholipase D. ChemistrySelect, 1, 4121-4125. [Wiley]

(2) 総説 (Reviews)

1. 加藤 晃代、中野 秀雄 (2016) 大腸菌無細胞／生細胞発現系によるモノクローナル抗体取得技法　細胞　48 (4), 167-171.

(3) その他 (Others)

1. 岩崎 雄吾 "3) 脂質, テルペノイド" (第5章 微生物の代謝)，応用微生物学 第3版 (横田 篤, 大西 康夫, 小川 順 編) 文永堂, pp128-131, (2016)

2. 山根 恒夫，中野 秀雄, 加藤 雅士, 岩崎 雄吾, 河原崎 泰昌, 志水 元亨: 新版 生物反応工学. 産業図書 (2016)

 

2015

(1) 原著論文 (Original Papers)

1. DeKosky, B. J., Kojima, T., Rodin, A., Charab, W., Ippolito, G., Ellington, A. D., and Georgiou, G. (2015) In-Depth Determination and Analysis of the Human Paired Heavy and Light Chain Antibody Repertoire. Nat. Med. 21, 86-91. [nature.com]

2. Mori, A., Hara, S., Sugahara, T., Kojima, T., Iwasaki, Y., Kawarasaki, Y., Sahara, T, Ohgiya, S., and Nakano, H. (2015) Signal peptide optimization tool for the secretion of recombinant protein from Saccharomyces cerevisiae. J. Biosci. Bioeng. 120, 518-525. [Science Direct]

3. Zhu, B., Mizoguchi, T., Kojima, T., and Nakano, H. (2015) Ultra-High-Throughput Screening of an In Vitro-Synthesized Horseradish Peroxidase Displayed on Microbeads Using Cell Sorter. PLOS ONE 10, e0127479. [PubMed]

4. Shen, X., Saburi, W., Gai, Z., Kato, K., Ojima-Kato, T., Yu, J., Komoda, K., Kido, Y., Matsui, H., Mori, H., and Yao, M. (2015) Strucutral analysis of α-glucosidase HaG provides new insights into substrate specificity and catalytic mechanism. Acta Crystallogr D Biol. Crystallogr. 1382-1391. [IUCr Journals]

5. Murakami, Y., Ojima-Kato, T., Saburi, W., Mori, H., Matsui, H., Tanabe, S., and Suzuki, T. (2015) Supplemental epilactose prevents metabolic disorders through uncoupling protein-1 induction in the skeletal muscle of mice fed high-fat diets. Br. J. Nutr. 114,1774-1783. [Cambridge University Press]

6. Ojima-Kato, T., Hashimura, D., Kojima, T., Minabe, S., and Nakano, H. (2015) In vitro generation of rabbit anti- Listeria monocytogenes monoclonal antibody using single cell based RT-PCR linked cell-free expression systems. J. Immunol. Methods 427, 58-65. [Science Direct]

7. Ojima-Kato T, Yamamoto N, Iijima Y, and Tamura H. (2015) Assessing the performance of novel software Strain Solution on automated discrimination of Escherichia coli serotypes and their mixtures using matrix-assisted laser desorption ionization-time of flight mass spectrometry. J. Microbiol. Methods 119, 233-8. [PubMed]

(2) 総説 (Reviews)

1. Kojima, T., Zhu, B., and Nakano, H. (2015) Construction of a DNA library on microbeads using whole genome amplification. Whole Genome Amplification, Methods in Molecular Biology, T. Kroneis (ed.) 1347, 87-100. [Springer]

 

2014

(1) 原著論文 (Original Papers)

1. Eba, C., Okano, A., Nakano, H., and Iwasaki, Y. (2014) A chromogenic substrate for solid-phase detection of phospholipase A 2 . Anal. Biochem. 447, 43-45. [Science direct]

2. Damnjanovic, J., Nakano, H., and Iwasaki, Y. (2014) Deletion of a dynamic surface loop improves stability and changes kinetic behavior of phosphatidylinositol-synthesizing Streptomyces phospholipase D. Biotechnol. Bioeng. 111, 674-82. [Wiley] Selected as "Spotlight"

3. Ninomiya, R., Zhu, B., Kojima, T., Iwasaki, Y., and Nakano, H. (2014) Role of disulfide bond isomerase DsbC, calcium ions, and hemin in cell-free protein synthesis of active manganese peroxidase isolated from Phanerochaete chrysosporium. J. Biosci. Bioeng. 117, 652-657. [Science direct]

4. Kelton, W., Mehta, N., Charab, W., Lee, J., Lee, C., Kojima, T., Kang, T. H., Georgiou, G. (2014) IgGA: A "Cross-Isotype" Engineered Human Fc Antibody Domain that Displays Both IgG-like and IgA-like Effector Functions. Chem. Biol. 21, 1603-1609. [Cell Press]

(2) 総説 (Reviews)

1. 中野 秀雄 (2014)　「免疫測定法」（小林、上田、三宅、荒川編）講談社 146-151.

2. 中野秀雄、兒島孝明 (2014) 試験管内タンパク質合成-化学便覧応用化学編　第7版- 丸善出版 512-515.

3. 兒島孝明、中野秀雄 (2014)「超微細生化学反応系」技術の最前線　化学と生物 52, 159-165.

4. 兒島孝明、中野秀雄 (2014) ビーズディスプレイ法を用いたDNA-転写因子間相互作用のハイスループット解析　バイオサイエンスとインダストリー 72, 306-308.

 

2013

(1) 原著論文 (Original Papers)

1. Damnjanovic, J., Nakano, H., and Iwasaki, Y. (2013) Simple and efficient profiling of phospholipids in phospholipase D-modified soy lecithin by HPLC with charged aerosol detection. J. Am. Oil Chem. Soc. 90, 951-957. [Springer]

2. Wada, Y., Matsubara, A., Uchikata, T., Iwasaki, Y., Morimoto, S., Kan, K., Ookura, T., Fukusaki, E., and Bamba, T. (2013) Investigation of β-Cryptoxanthin Fatty Acid Ester Compositions in Citrus Fruits Cultivated in Japan. Food Nutr. Sci., 4, 98-104. [Scientific Research]

(2) 総説 (Reviews)

1. Damnjanovic, J., and Iwasaki, Y. (2013) Phospholipase D as a catalyst: application in phospholipid synthesis, molecular structure and protein engineering. J. Biosci. Bioeng. 116, 271-280. [Science direct]

2. 岩崎雄吾(2013) 放線菌ホスホリパーゼDの改変とリン脂質合成への応用 オレオサイエンス 13, 465-469.

 

2012

(1) 原著論文 (Original Papers)

1. Takemori, D., Yoshino, K., Eba, C., Nakano, H., and Iwasaki, Y. (2012) Extracellular production of phospholipase A2 from Streptomyces violaceoruber by recombinant Escherichia coli. Protein Expr. Purif. 81, 145-150. [Science direct]

2. Wang, P., Kojima, T., Kobayashi, T., and Nakano, H. (2012) Comprehensive Analysis of the DNA-Binding Specificity of an Aspergillus nidulans Transcription Factor, AmyR, by using a Bead Display System. Biosci. Biotechnol. Biochem. 76, 1128-1134. [J-STAGE]

3. Damnjanovic, J., Takahashi, R., Suzuki, A., Nakano, H., and Iwasaki, Y. (2012) Improving thermostability of phosphatidylinositol-synthesizing Streptomyces phospholipase D. Protein Eng. Des. Sel. 25, 415-424. [Oxford journals]

4. Kojima, T., Ohuchi, S., Ito, Y., Nakano, H. (2012) High-throughput screening method for promoter activity using bead display and a ligase ribozyme. J. Biosci. Bioeng. 114, 671-676. [Science direct]

(2) 総説 (Reviews)

1. 中野秀雄, 兒島孝明 (2012) 無細胞蛋白質合成系の利用技術の新展開-バイオ医薬品製造の効率化と生産基材の開発-（山口照英監修). 193-201. シーエムシー出版.

 

2011

(1)原著論文 (Original Papers)

1. Kojima, T., Nagao, N., Ando, D., Ojima, T., Kawarasaki, Y., Kobayashi, I., Nakajima, M. and Nakano, H. (2011) Emulsion Culture: A Miniaturized Library Screening System Based on Micro-droplets in an Emulsified Medium. J. Biosci. Bioeng. 112, 299-303. [Science direct]

2. Kojima, T., Karasawa, S., Miyawaki, A., Tsumuraya, T., Fujii, I. (2011) Novel screening system for protein-protein interactions by bimolecular fluorescence complementation in Saccharomyces cerevisiae. J Biosci Bioeng. 111, 397-401. [Science direct]

3. Wada, Y., Matsubara, A., Uchikata, T., Iwasaki, Y., Morimoto, S., Kan, K., Okura, T., Fukusaki, E., and Bamba, T. (2011) Metabolic profiling of beta-cryptoxanthin and its fatty acid esters by supercritical fluid chromatography coupled with triple quadrupole mass spectrometry. J. Sep. Sci. 34, 3546-3552. [Wiley]

(2) 総説 (Reviews)

1. Kojima, T. and Nakano, H. (2011) GLOBE:Analysis of DNA-Protein Interaction Analysis. PCR Protocols, Methods in Molecular Biology, J. Park (ed.), 687, 307-317. [SpringerLink]

 

2010

(1) 原著論文 (Original Papers)

1. Ozaki, A., Masayama, A., Nakano, H. and Iwasaki, Y. (2010) Synthesis of phosphatidylinositols having various inositol stereoisomers by engineered phospholipase D. J. Biosci. Bioeng. 109, 337-340. [Science direct]

2. Sabrina, Y., Ali, M. and Nakano, H. (2010) In vitro generation of anti-hepatitis B monoclonal antibodies from a single plasma cell using single-cell RT-PCR and cell-free protein synthesis. J. Biosci. Bioeng. 109, 75-82. [Science direct]

3. Gan, R., Furuzawa, S., Kojima, T., Kanie, K., Kato, R., Okochi, M., Honda, H. and Nakano, H. (2010) Directed Evolution of Angiotensin II-inhibiting Peptides Using Microbeads Display. J. Biosci. Bioeng. 109, 411-417. [Science direct]

4. Kojima, T., Hashimoto, Y., Kato, M., Kobayashi, T. and Nakano, H. (2010) High-throughput Screening of DNA Binding Sites for Transcription Factor AmyR from Aspergillus nidulans Using DNA Beads Display System. J. Biosci. Bioeng. 109, 519-525. [Science direct]

5. Tanaka, T., Isezaki, T., Nakano, H. and Iwasaki, Y. (2010) Synthesis of phospholipids containing polyunsaturated fatty acids by phospholipase A2-mediated esterification with food-compatible reagents. J. Oleo Sci. 59, 375-380. [J-STAGE]

6. Kamiya T., Ojima T., Sugimoto K., Nakano H. and Kawarasaki Y. (2010) Quantitative Y2H screening: cloning and signal peptide engineering of a fungal secretory LacA gene and its application to yeast two-hybrid system as a quantitative reporter. J Biotechnol. 146, 151-9. [PubMed]

7. Ikeuchi A., Nakano H., Kamiya T., Yamane T. and Kawarasaki Y. (2010) A method for reverse interactome analysis: High-resolution mapping of interdomain interaction network in Dam1 complex and its specific disorganization based on the interaction domain expression. Biotechnol. Progress. 26, 945-53. [WILEY]

 

2009

(1) 原著論文 (Original Papers)

1. Masayama, A., Tsukada, K., Ikeda, C., Nakano, H., and Iwasaki, Y.　(2009)　Isolation of phospholipase D mutants having phosphatidylinositol- synthesizing activity with positional specificity on myo-inositol. Chembiochem. 10, 559-564. [PubMed]

2. Iwasaki, Y., Masayama, A., Mori, A., Ikeda C. and Nakano, H. (2009)　Composition analysis of positional isomers of phosphatidylinositol by high-performance liquid chromatography. J. Chromatogr. A. 1216, 6077-6080. [PubMed]

(2) 総説 (Reviews)

1. 昌山　敦、鈴木　淳巨、岩崎　雄吾　(2009) ホスホリパーゼDの機能改変. 生物工学会誌、87、274-276.

2. 兒島　孝明、甘　　睿、中野　秀雄　(2009) エマルジョンPCRが拓く分子間相互作用ハイスループットスクリーニング. 生物工学会誌、87、283-286.

(3) その他 (Others)

1. Nakano, H. and Subrina, Y.:High-throughput generation of monoclonal antibodies from single B cells using RT-PCR and a cell-free protein synthesis system. Proceeding of APBioChEC'09, Kobe 2009, J. Biosci. Bioeng. 108, S1, S22-23. [Science direct]

2. Kojima, T., Gan, R., Yamanaka, Y. and Nakano, H.:Beads display:Genatype-phenotype coupling screening of DNA molecules displayed on microbeads by using emulsion PCR and in vitro protein synthesis. Proceeding of APBioChEC'09, Kobe 2009, J. Biosci. Bioeng. 108, S1, S105-106. [Science direct]

 

2008

(1) 原著論文 (Original Papers)

1. Masayama, A., Takahashi, T., Tsukada, K., Nishikawa, S., Takahashi, R., Adachi, M., Koga, K., Suzuki, A., Yamane T., Nakano, H., and Iwasaki, Y. (2008) Streptomyces phospholipase D mutants with altered substrate specificity capable of phosphatidylinositol synthesis. Chembiochem., 9, 974-981. [PubMed]

2. Deng, L., Nakano, H. and Iwasaki, Y. (2008) Direct separation of monoacylglycerol isomers by enantioselective high-performance liquid chromatography. J. Chromatogr. A., 1198-1199C, 67-72 [PubMed]

3. Gan, R, Yamanaka, Y, Kojima, T, and Nakano, H. (2008) Microbeads display of proteins using emulsion PCR and cell-free protein synthesis. Biotechnol. Prog., 24, 1107-1114 [WILEY]

(2) 総説 (Reviews)

1. 岩崎雄吾、昌山敦、中野秀雄 (2008) ホスホリパーゼＤによるリン脂質の変換-微生物によるものづくり―化学法に代わるホワイトバイオテクノロジーの全て―（植田充美監修）. 100-105. シーエムシー出版.

(3) その他 (Others)

1. Hideo Nakano, Yusuke Sugihara, and Hideki Matsuda:Engineering of cellulases using a cell-free protein synthesis system. Proceeding of Biotechnology of Lignocelulose Degradation and Biomass Utilization, Mie Bioforum 2008, pp460-466

 

2007

(1) 原著論文 (Original Papers)

1. Deng, L., Nakano, H. and Iwasaki, Y. (2007) Direct separation of regioisomers and enantiomers of monoacylglycerols by tandem column high-performance liquid chromatography. J. Chromatogr. A. 1165, 93-9. [PubMed]

(2) 総説 (Reviews)

1. Rungpragayphan, S., Yamane, T. and Nakano, H. (2007) SIMPLEX: single-molecule PCR-linked in vitro expression: a novel method for high-throughput construction and screening of protein libraries. Methods Mol. Biol., 79-94. [PubMed]

2. Koga, Y., Yamane, T. and Nakano, H. (2007) Creation of novel enantioselective lipases by SIMPLEX. Methods Mol. Biol., 165-182. [PubMed]

3. 兒島孝明、中野秀雄(2007) 無細胞タンパク質合成系を用いた進化分子工学システムの構築と応用. 生化学、79、239-246.

(3) その他 (Others)

1. 中野秀雄（編集）(2007) 「ナノバイオ大事典」テクノシステム.

 

2006

(1) 原著論文 (Original Papers)

1. Kojima, T., Yamane, T. and Nakano, H. (2006) In vitro selection of DNA binding sites for transcription factor, PhaR, from Paracoccus denitrificans using genetic library on microbeads and flow cytometry. J. Biosci. Bioeng., 101, 440-444. [J-STAGE]

2. Muhamad Ali, Hitomi, K. and Nakano, H. (2006) Generation of Monoclonal Antibodies Using Simplified Single-Cell Reverse Transcription-Polymerase Chain Reaction and Cell-Free Protein Synthesis. J. Biosci. Bioeng., 101, 284-286 [J-STAGE]

3. Piyatheerawong, W., Yamane, T., Nakano, H. and Iwasaki, Y. (2006) Enzymatic Preparation of Enantiomerically Pure sn-2,3-Diacylglycerols: A Stereoselective Ethanolysis Approach. J. Am. Oil Chem. Soc., 83, 603-607. [Springer Link]

4. Jiang, X.P., Suzuki, H., Hanai, Y., Wada, F., Hitomi, K., Yamane, T. and Nakano, H.(2006) A Novel Strategy for Generation of Monoclonal Antibodies from Single B Cells Using RT-PCR Technique and in Vitro Expression. Biotechnol. Prog., 22, 979-988. [ACS Publication]

(2) 総説 (Reviews)

1. 吉村千里、中野秀雄(2006) 微細形状を利用した高速高感度免疫化学検出. 電気学会論文誌E、126、486-491.

2. 中野秀雄(2006) SIMPLEX法. バイオプロダクション、化学工学会バイオ部会編（コロナ社）52-56

 

 2005

(1) 原著論文 (Original Papers)

1. Kojima, T., Takei, Y., Ohtsuka, M., Kawarasaki, Y., Yamane, T. and Nakano, H. (2005) PCR amplification form single DNA molecules on magnetic beads in emulsion: application for high-throughput screening of transcription factor targets. Nucleic Acids Res., 33, e150. [PubMed]

2. Piyatheerawong, W., Iwasaki, Y. and Yamane, T. (2005) Direct separation of regio- and enantiomeric isomers of diacylglycerols by a tandem column high-performance liquid chromatography. J. Chromatogr. A, 1068, 243-248. [PubMed]

3. Yamane, T., Ikeda, Y., Nagasaka, T. and Nakano, H. (2005) Enhanced cell-free protein synthesis using a S30 extract from Escherichia coli grown rapidly at 42 °C in an amino acid enriched medium. Biotechnol. Prog., 21, 608-613. [Publisher's website]

4. Muhamad, Ali, Suzuki, H., Fukuba, T., Jiang, X., Nakano, H. and Yamane, T. (2005) Improvements in the cell-free production of functional antibodies using cell extract from protease-deficient Escherichia coli mutant. J. Biosci. Bioeng., 99, 181-186. [PubMed]

5. Kato, R., Nakano, H., Konishi, H., Kato, K., Koga, Y., Yamane T., Kobayashi, T., and Honda, H.(2005) Novel strategy for protein exploration: High-throughput screening assisted with fuzzy neural network. J. Mol. Biol., 351, 683-692. [PubMed]

 

 2004

(1) 原著論文 (Original Papers)

1. Ikeuchi, A., Sasaki, Y., Kawarasaki, Y. and Yamane, T. (2004) Exhaustive identification of interaction domains using a high-throughput method based on two-hybrid screening and PCR-convergence: Molecular dissection of a kinetochore subunit Spc34p. Nucleic Acids Research, 31(23), 6953-6962. [PubMed]

2. Kojima, T., Nishiyama, T., Maehara, A., Ueda, S., Nakano, H. and Yamane, T. (2004) Expression profiles of polyhydroxyalkanoate synthesis-related genes in Paracoccus denitrificans. Journal of Bioscience and Bioengineering, 97, 45-53. [SienceDirect]

3. Piyatheerawong, W., Iwasaki, Y., Xu, X. and Yamane, T. (2004) Dependency of water concentration on ethanolysis of trioleoylglycerol by lipases. Journal of Molecular Catalysis B: Enzymatic, 28, 19-24. [SienceDirect]

4. Iwasaki, Y., Nishikawa, S., Tsuneda, M., Takahashi, T. and Yamane, T. (2004) Detection of phospholipase D on solid materials. Analytical Biochemistry, 329, 157-159. [PubMed]

5. Rungpragayphan, S., Haba, M., Nakano, H. and Yamane, T. (2004) Rapid screening for affinity-improved scFvs by means of single-molecule-PCR-linked in vitro expression. Journal of Molecular Catalysis B: Enzymatic, 28, 223-228. [SienceDirect]

6. Takagi, Y., Yasuda, R., Maehara, A. and Yamane, T. (2004) Microbial synthesis and characterization of polyhydroxyalkanoates with fluorinated phenoxy side groups from Pseudomonas putida. European Polymer Journal, 40, 1551-1557. [SienceDirect]

7. Kinpara, T., Mizuno, R., Murakami, Y., Kobayashi, M., Yamaura, S., Hasan, Q., Morita, Y., Nakano, H., Yamane, T. and Tamiya, E. (2004) A picoliter chamber array for cell-free protein synthesis. European Polymer Journal, 40, 1551-1557. [J-STAGE]

(2) 総説（Reviews)

1. Nakano, H., Kawarasaki, Y. and Yamane, T. (2004) Cell-free protein synthesis systems: Increasing their performance and applications. Advances in Biochemical Engineering/Biotechnology, 90, Recent Progress of Biochemical and Biomedical Engineering in Japan I., ed. by Takeshi Kobayashi, Chapter 6(pp. 135-149), Springer-Verlag Berlin & Heidelberg.

2. Iwasaki, Y. and Yamane, T. (2004) Enzymatic synthesis of structured lipids. Advances in Biochemical Engineering/Biotechnology, 90, Recent Progress of Biochemical and Biomedical Engineering in Japan I., ed. by Takeshi Kobayashi, Chapter 7(pp. 151-171), Springer-Verlag Berlin & Heidelberg.

(3) その他（Others)

1. 岩崎雄吾（分担）:「油化学辞典 脂質・界面活性剤」（日本油化学会編）　丸善（2004）

 

 2003

(1) 原著論文 (Original Papers)

1. Yasuaki Kawarasaki, Yasuhiro Yamada, Maki Ichimori, Tomoya Shinbata, Katsunori Kohda, Hideo Nakano, and Tsuneo Yamane: Stabilization of affinity-tagged recombinant protein during/after its production in a cell-free system using wheat-germ extract. Journal of Bioscience and Bioengineering, 95(3), 209-214(2003). [SienceDirect]

2. Suang Rungpragayphan, Hideo Nakano, and Tsuneo Yamane: PCR-linked in vitro expression: A novel system for high-throughput construction and screening of protein libraries. FEBS letters, 540, 147-150 (2003). [PubMed]

3. Denfu Chen, Yasuaki Kawarasaki, Hideo Nakano, and Tsuneo Yamane: Cloning and in vitro and in vivo expressions of plant glutathione S-transferase Zeta class genes. Journal of Bioscience and Bioengineering, 95(6), 594-600(2003). [SienceDirect]

4. Chie Miyazaki-Imamura, Kazuyo Oohira, Ritsuko Kitagawa, Hideo Nakano, Tsuneo Yamane, and Haruo Takahashi: Improvement of H 2 O 2 stability of manganese peroxidase by combinatorial mutagenesis and high-throughput screening using in vitro expression with protein disulfide isomerase. Protein Eng. 16(6):423-428 (2003) [PubMed]

5. Yuichi Koga, Katsuya Kato, Hideo Nakano, and Tsuneo Yamane: Inverting enantioselectivity of Burkholderia cepacia KWI-56 lipase by combinatorial mutation and high-throughput screening using single-molecule PCR and in vitro expression. Journal of Molecular Biology, 331(3), 585-592(2003). [PubMed]

6. Yugo Iwasaki, Yukiko Mizumoto, Takahiro Okada, Takaya Yamamoto, Kentaro Tsutsumi and Tsuneo Yamane: An aqueous suspension system for phospholipase D-mediated synthesis of phosphatidylserine without toxic organic solvent. Journal of the American Oil Chemists' Society, 80, 653-657 (2003). [Publisher's website]

7. Akinori Ikeuchi, Yasuaki Kawarasaki, Tomoya Shinbata, and Tsuneo Yamane: Chimeric Gene Library Construction by a Simple and Highly Versatile Method Using Recombination-Dependent Exponential Amplification: Biotechnology Progress, 19(5), 1460-1467(2003). [Publisher's website]

(2) 総説（Reviews)

1. 山根恒夫： 無細胞タンパク質合成系。 農芸化学の事典（鈴木昭憲、荒井綜一編集），1.3.4節(pp. 35-37), 朝倉書店 (2003).

2. Hideo Nakano and Tsuneo Yamane: In vitro expression of proteins with disulfide bridges and its application for high-throughput screening system. "Cell-Free Protein Expression" ed. by James R. Swartz, Chap. 14 (pp. 117-124), Springer-Verlag GmbH & Co. KG, Berlin/Heidelberg, (2003).

3. 高木康雄、山根恒夫： 微生物産系ＰＨＡ．「生分解性プラスチックの改質技術と成型加工における課題と対策」、技術情報協会、第１章「生分解性プラスチックの分解速度の設計およびコント ロール技術」、第５節(pp. 61-78）(2003).

 

 2002

(1) 原著論文 (Original Papers)

1. Xiuping Jiang, Kazuyo Oohira, Yugo Iwasaki, Hideo Nakano, Shigeyuki Ichihara, and Tsuneo Yamane: Diminishment of protein degradation by using protease-deficient mutants in Eshcerichia coli cell-free protein synthesis. Journal of Bioscience and Bioengineering., 93 (2), 151-156 (2002). [SienceDirect]

2. Junhao Yang, Yuichi Koga, Hideo Nakano, and Tsuneo Yamane: Modifying the chain length selectivity of the lipase from Burkholderia cepacia KWI-56 through in vitro combinatorial mutagenesis in the substrate binding site. Protein Engineering, 15(2), 101-106(2002). [PubMed]

3. Xiuping Jiang, Yuji Ookubo, Ikuo Fujii, Hideo Nakano, and Tsuneo Yamane: Expression of Fab fragment of catalytic antibody 6D9 in an Escherichia coli coupled transcription/translation system. FEBS Letters, 514, 290-294 (2002). [PubMed]

4. Yasuaki Kawarasaki, Yoko Sasaki, Akinori Ikeuchi, Shoji Yamamoto and Tsuneo Yamane: A method for a functional mapping of protein-protein binding domain by preferential amplification of the shortest amplicon using PCR. Analytical Biochemistry, 303, 34-41 (2002). [PubMED]

5. Suang Rungpragayphan, Yasuaki Kawarasaki, Takao Imaeda, Katsunori Kohda, Hideo Nakano, and Tsuneo Yamane: High- throughput, cloning-independent protein library construction by combining single-molecule DNA amplification with in vitro expression. Journal of Molecular Biology, 318, 395-405 (2002). [PubMed]

6. Yuichi Koga, Kouei Kobayashi, Hideo Nakano, and Tsuneo Yamane: In vitro construction and screening of Burkholderia cepacia lipase library by using single-molecule PCR and cell-free protein synthesis. Journal of Bioscience and Bioengineering, 94, 84-86 (2002). [SienceDirect]

7. Yugo Iwasaki, Wanda Brown and Ching T. Hou: Biosynthetic pathway of diepoxy bicyclic fatty acids from linoleic acid by Clavibacter sp. ALA2. Journal of the American Oil Chemists' Society. 79, 369-372 (2002) [Publisher's website]

8. Akira Maehara, S. Taguchi, Tatsuaki Nishiyama, Tsuneo Yamane and Y. Doi: A Repressor Protein, PhaR, Regulates Polyhydroxyalkanoate (PHA) Synthesis via Its Direct Interaction With PHA. Journal of Bacteriology, 184 , 3992 - 4002, (2002). [PubMed]

9. Shunsaku Ueda, K. Sano, D. Gao, N. Tomihari, Tsuneo Yamane and I. Endo: Purification and properties of D-(－)-3-hydroxybutyrate oligomer hydrolase of Paracoccus denitrificans , FEMS Microbiology Letters. 206, 179 - 184 (2002). [PubMed]

10. Roxana Irimescu, Yugo Iwasaki, and Ching T. Hou: Study of TAG Ethanolysis to 2-MAG by Immobilized Candida antarctica Lipase and Synthesis of Symmetrically Structured TAG. Journal of the American Oil Chemists' Society, 79, 879-883 (2002). [Publisher's website]

11. Hideo Nakano, Reiko Okumura, Chinatsu Goto, and Tsuneo Yamane: In vitro combinatorial mutagenesis of the 65th and 222nd positions of the green fluorescent protein of Aequarea victoria. Biotechnology and Bioprocess Engineering, 7, 311-315(2002).

12. Shanmuga Sundara Raj, Eiji Kibushi, Tatsuhiro Kurasawa, Atsuo Suzuki, Takashi Yamane, Shoji Odani, Yugo Iwasaki, Tsuneo Yamane, and Tamaichi Ashida: Crystal structure of bovine trypsin and wheat germ trypsin inhibitor (I-2b) complex (2:1) at 2.3 A resolution. Journal of Biochemistry, 132(6), 927-933(2002). [PubMed]

(2) 総説（Reviews)

1. 中野秀雄，河原崎泰昌，山根恒夫： 無細胞タンパク質合成系の進歩と応用． 現代化学，2002年1月号，66-72(2002).

2. 山根恒夫： 微生物，酵素，および遺伝子の関与する反応の工学的研究． 生物工学会誌，80(1), 10-21(2002).

3. 山根恒夫，中野秀雄： 無細胞蛋白質合成系による新機能分子の創出．化学と生物、40(8)、533-538　(2002)

(3)単行本 (Books and Monographs)

1. Yugo Iwasaki and Tsuneo Yamane: Phospholipases in enzyme engineering of phospholipids for food, cosmetics and medical applications. In "Lipid Biotechnology" ed. by Tsung Min Kuo and Harold W. Gardner, Chap. 20 (pp. 417-431), Marcel Dekker, Inc., New York, (2002).

2. 山根恒夫: 生物反応工学(第3版). 産業図書(株), (2002).

3. 山根恒夫，中野秀雄，河原崎泰昌： 実験例　ＰＣＲ法による生体組織からの遺伝子増幅．生物工学実験書（改訂版），3.3,日本生物工学会編，培風館(2002).

4. 山根恒夫：無細胞タンパク質合成系。農芸化学の事典，1.3.4, 朝倉書店(2002).

5. 山根恒夫：流加培養系における基質流加法。「バイオプロセスシステムズエンジニアリング」、第５章第１節(pp. 115-125),（株）シーエムシー出版、(2002).

6. 山根恒夫、上田俊策、前原　晃： 微生物を利用する生分解性プラスチック合成。 「グリーンプラスチック最新技術」(井上義夫監修)、第６章(pp. 71-83)、（株）シーエムシー出版、(2002).

 

 2001

（１）原著論文 (Original Papers)

1. Yugo Iwasaki, Mamoru Yasui, Takuma Ishikawa, Roxana Irimescu, Kazuhiko Hata and Tsuneo Yamane: Optical resolution of asymmetric triacylglycerols by chiral-phase high performance liquid chromatography. Journal of Chromatography A, 950(1/2), 111-118 (2001). [PubMed]

2. Roxana Irimescu, Kazuhiko Hata, Yugo Iwasaki and Tsuneo Yamane: Comparison of acyl doners for lipase-catalyzed production of 1,3-dicapryloyl-2-eicosapentaenoylglycerol. Journal of the American Oil Chemists' Society, 78(1), 65-70(2001). [Publisher's website]

3. Xiuping Jiang, Hideo Nakano, Takanori Kigawa, Takashi Yabuki, Shigeyuki Yokoyama, Douglas S. Clark and Tsuneo Yamane: Dosage effect of minor arginyl- and isoleucyl-tRNAs on protein synthesis in Escherichia coli in vitro coupled transcription/translation system. Journal of Bioscience and Bioengineering, 91(1), 53-57(2001). [SienceDirect]

4. Roxana Irimescu, Kiyomi Furihata, Kazuhiko Hata, Yugo Iwasaki and Tsuneo Yamane: Utilization of reaction medium-dependent regiospecificity of Candida antarctica lipase (Novozym 435) for synthesis of 1,3-dicapryloyl- 2-docosahexaenoyl (or eicosapentaenoyl) glycerol. Journal of the American Oil Chemists' Society, 78(3), 285-289(2001). [Publisher's website]

5. Dai Gao, Akira Maehara, Tsuneo Yamane, and Shunsaku Ueda: Identification of the intracellular polyhydroxyalkanoate depolymerase gene of Paracoccus denitrificans and the region essential for its expression. FEMS Microbiology Letters, 196(2), 159-164 (2001). [PubMed]

6. Roxana Irimescu, Kiyomi Furihata, Kazuhiko Hata, Yugo iwasaki, and Tsuneo Yamane: Two-step enzymatic synthesis of DHA-rich symmetrically structured triacylglycerols via 2-monoacylglycerols. Journal of the American Oil Chemists' Society, 78(7), 743-748 (2001). [Publisher's website]

7. Akira Maehara, Yoshiharu Doi, Tatsuaki Nishiyamna, Yasuo Takagi, Shunsaku Ueda, Hideo Nakano, and Tsuneo Yamane: PhaR, a protein of unknown function conserved among short-chain-length polyhydroxyalkanoic acids producing bacteria, is a DNA-binding protein and represses Paracoccus denitrificans phaP expression in vitro . FEMS Microbiology Letters, 200(1), 9-15(2001). [PubMed]

(2) 総説(Reviews)

1. 山根恒夫，岩崎雄吾：構造脂質の酵素合成. オレオサイエンス、1(8), 825-833(2001).

2. 河原崎泰昌，中野秀雄、山根恒夫：無細胞タンパク質合成系を用いたコンビナトリアル・バイオエンジニアリング.

3. Akira Maehara, Tsuneo Yamane, Seiichi Taguchi, and Yoshiharu Doi: Molecular characterization of a regulatory protein (PhaR) involved in PHA biosynthesis. RIKEN Review, 42(12), 77-80(2001).

(3) 単行本(Books and Monographs)

1. Tsuneo Yamane: Choice of solvent for enzymatic reaction in organic solvent. In "Handbook of Solvents" ed. by Dr. George Wypych, Chap. 14.4.3 (pp. 872-880), ChemTec Laboratories, Inc., Toronto-Scarborough, (2001).

2. Tsuneo Yamane: Solvent-free biotransformations of lipids. In " Enzymes in Nonaqueous Solvents " ed. by Jenya Vulfson, Peter J. Halling, and Herbert L. Holland (in the series, Methods in Biotechnology ), Chap.38(pp. 509-516), The Humana Press Inc., (2001).

3. 山根恒夫： IV. 無細胞系. 組替えタンパク質生産法（生物化学実験法45）(塚越規弘編著)、pp. 159-180,（株）学会出版センター、(2001).

4. 山根恒夫：油脂領域におけるバイオリアクターの利用． 「油脂化学便覧」第４版，6.2.4（社）日本油化学会編，丸善（株）(2001).

 

 2000

(1) 原著報文 (Original Papers)

1. Hideo Nakano,  Koei Kobayashi, Shoji Ohuchi, Satoshi Sekiguchi, and Tsuneo Yamane: Single-step single-molecule PCR using a single primer and hot-startable DNA polymerase. Journal of Bioscience and Bioengineering, 90(4), 456-458 (2000). [SienceDirect]

2. Yugo Iwasaki, Tatsuaki Nishiyama, Yasuaki Kawarasaki, Hideo Nakano and Tsuneo Yamane: Importance of disulfide bridge formation on folding of phospholipase D from Streptomyces antibioticus . Journal of Bioscience and Bioengineering, 89, 506-508 (2000). [SienceDirect]

3. Yasuaki Kawarasaki, Satoru Kasahara, Natsuko Kodera, Satoshi Sekiguchi, Hideo Nakano, and Tsuneo Yamane: A trimmed viral translation enhancing sequence for a rapid in vitro gene expression using wheat-germ extract. Biotechnology Progress, 16, 517-521(2000).
   [PubMed]

4. Roxana Irimescu, Mamoru Yasui, Yugo Iwasaki, Nobuyoshi Shimizu, and Tsuneo Yamane: Enzymatic synthesis of 1,3-dicapryloyl-2-eicosapentaenoylglycerol. Journal of the American Oil Chemists' Society, 77, 501-506(2000). [Publisher's website]

5. Hiroyuki Shibazaki and Tsuneo Yamane: Avoidance of solidification of sesame oil at low temperature by self-interesterification with immobilized lipase. Bioscience, Biotechnology and Biochemistry, 64, 1011-1015 (2000).

6. Junhao Yang, Koei Kokayashi, Yugo iwasaki, Hideo Nakano, and Tsuneo Yamane: In vitro analysis of a disulfide bridge and a calcium binding site in activation of Pseudomonas sp. strain KWI-56 lipase. Journal of Bacteriology, 182(2), 295-302(2000). [PubMed]

7. Jiann-Tsyh Lin, Karen M. Lew, Jennifer M. Chen, Yugo Iwasaki and Thomas A. McKeon:Metabolism of 1-acyl-2-oleoyl- sn -glycero-3-phosphoethanolamine in castor oil biosynthesis. Lipids, 35, 481-486 (2000). [PubMed]

(2) 総説 (Reviews)

1. Yugo Iwasaki and Tsuneo Yamane: Enzymatic synthesis of structured lipids. Journal of Molecular Catalysis, B: Enzymatic, 10(1-3), 129-140 (2000).

2. Tsuneo Yamane: Lipase-catalyzed synthesis of structured triacylglycerols. - Monitoring the reaction and increasing the yields - In "Enzymes in Lipid Modification" ed. by Uwe Bornscheuer, Chap.9 (pp148-169) Viley-VCH, Weinheim, Germany, 2000.

3. Tsuneo Yamane: Solvent-free Biotransformation of Lipids. In "Methods in Nonaqueous Enzymology" ( a volume of "Methods in Biotechnology" series), ed. By J. Vulfson, Peter Halling, and John Woodley, Humana Inc.,99.509-516, 2000.

4. 河原崎泰昌、山根恒夫：高効率化の進む最近の生体外遺伝子発現系．化学と生物、38(4), 220-222(2000).

5. 山根恒夫, 岩崎雄吾：機能性脂質とバイオテクノロジー．バイオサイエンスとインダストリー．58(12), 839-844(2000).

6. 山根恒夫：IV. 無細胞系．組換えタンパク質生産法（生物化学実験法），塚越規弘編著，（株）学会出版センター，(2000)．

7. 中野秀雄：Good-bye　"大腸菌".　無細胞蛋白質合成系の挑戦。　バイオサイエンスとバイオインダストリー、58（８）、541-545（2000).

 

 1999

（１）原著報文(Original Papers)

1. Atsuo Suzuki, Kouji Kakuno, Yugo Iwasaki, Tsuneo Yamane andTakashi Yamane: Crystallization and preliminary X-ray crystallographic studies of phospholipase D from Streptomyces antibioticus . Acta Crystallographica, D55, 317-319 (1999). [PubMed]

2. Jeong-Jun Han, Yugo Iwasaki and Tsuneo Yamane:Monitoring of lipase-catalyzed transesterification between eicosapentaenoic acid ethyl ester and tricaprylin by silver ion high performance liquid chromatography and high temperature gas chromatography. Journal of the American Oil Chemists' Society, 76(1), 31-39 (1999). [Publisher's website]

3. Chiaki Ogino, Yukinari Negi, Toshiko Matumiya, Koichi Nakaoka, Akihiko Kondo, Shun'ichi Kuroda, Shinji Tokuyama, Ushio Kikkawa, Tsuneo Yamane, and Hideki Fukuda: Purification, Chracterization, and Sequence Determination of phospholipase D secreted by Streptoverticillium chinamoneum . Journal of Biochemistry, 125(2), 263-269(1999). [PubMed]

4. Akira Maehara, Shunsaku Ueda, Hideo Nakano, and Tsuneo Yamane: Analyses of a Polyhydroxyalkanoic acid Granule-Associated 16-Kilodalton Protein and Its Putative Regulator in the pha Locus of Paraccocus denitrificans . Journal of Bacteriology, 181(9), 2914-2921(1999). [PubMed]

5. Hideo Nakano, Marco Wieser, Byungserk Hurh, Takahiro Kawai, Toyokazu Yoshida, Tsuneo Yamane and Toru Nagasawa: Purification, characterization and gene cloning of 6-hydroxynicotinate 3-monooxygenase from Pseudomonas fluorescens TN5. European Journal of Biochemistry, 260(1), 120-126(1999). [PubMed]

6. Yasuo Takagi, Mitsuya Hashii, Akira Maehara, and Tsuneo Yamane: Biosynthesis of polyhydroxyalkanoate with a thiophenoxy side group obtained from Pseudomonas putida . Macromolecules, 32(25), 8315-8318(1999).

7. Junhao Yang, Koei Kobayashi, Hideo Nakano, Junko Tanaka, Takuya Nihira, Yasuhiro Yamada, and Tsuneo Yamane: Modulator-mediated synthesis of active lipase of Pseudomonas sp. 109 by Escherichia coli cell-free coupled transcription/translation system. Journal of Bioscience and Bioengineering, 88(6), 605-609(1999). [ScienceDirect]

8. Lee Yong-Woo and Tsuneo Yamane: Estimation of residual biomass, PHB, and nutrient concentrations by supplied amount of ammonia solution in fermentation of Alcaligenes latus . Journal of Microbiology and Biotechnology, 9(5), 554-561(1999).

9. Jeong-Jun Han and Tsuneo Yamane: Enhancement of both reaction yield and rate of the synthesis of structured triacylglycerol containing eicosapentaenoic acid under vacuum with water activity control. Lipids, 34(9), 34, 989-995(1999). [PubMed]

10. Yugo Iwasaki, Satoko Horiike, Kayoko Matsushita, and Tsuneo Yamane: Location of the catalytic nucleophile of phospholipase D of Streptomyces antibioticus in the C-terminal half domain. European Journal of Biochemistry, 264(2), 577-581(1999). [PubMed]

11. Roxana Rosu, Mamoru Yasui, Yugo Iwasaki, Nobuyoshi Shimizu, and Tsuneo Yamane: Enzymatic synthesis of symmetrical 1,3-diacylglycerols by direct esterification of glycerol in solvent-free system. Journal of the American Oil Chemists' Society, 76(7), 839-843(1999). [Publisher's website]

12. Yugo Iwasaki, Jeong-Jun Han, Miho Narita, Roxana Rosu and Tsuneo Yamane: Enzymatic synthesis of structured lipids from single cell oil of high docosahexaenoic acid content. Journal of the American Oil Chemists' Society, 76(5), 563-569(1999). [Publisher's website]

13. Jeong-Jun Han, Yugo Iwasaki and Tsuneo Yamane: Use of isopropanol as a modifier in a hexane-acetonitrile based mobile phase for the silver iron HPLC separation of positional isomers of triacylglycerols containing long chain polyunsaturated fatty acids. Journal of High Resolution Chromatography, 22(6), 357-361(1999).

14. Hideo Nakano, Tomoya Shinbata, Reiko Okumura, Satoshi Sekiguchi, Masatoshi Fujishiro and Tsuneo Yamane:Efficient coupled transcription/translation for PCR template by a hollow fiber membrane bioreactor. Biotechnology and Bioengineering, 64(2), 194-199(1999). [PubMed]

（２）総説(Reviews)

1. Tsuneo Yamane: Monoacylglycerols. Bioprocess Technology: Fermentation, Biocatalysis, and Bioseparation (Ed. M. C. Flickinger, and S. W. Drew, eds.), John Wiley & Sons, Inc., New York, Vol. 4, p.1810-1818(1999).

 

 1998

（１）原著報文(Original Papers)

1. Yugo Iwasaki, Yukiko Tsubouchi, Akiko Ichihashi, Hideo Nakano, Tomoko Kobayashi, Hiroh Ikezawa, and Tsuneo Yamane: Two distinct phosphatidylinositol-specific phospholipase Cs from Streptomyces antibioticus. Biochimica Biophysica Acta, 1391, 52-66(1998). [PubMed]

2. Yasuaki Kawarasaki, Hideo Nakano and Tsuneo Yamane: Phosphatase-immunodepleted cell-free protein synthesis system. Jounal of Biotechnology, 61(3), 199-208(1998). [PubMed]

3. Roxana Rosu, Yugo Iwasaki, Nobuyoshi Shimizu, Nobushige Doisaki, and Tsuneo Yamane: Enzymatic synthesis of glycerides from DHA-enriched PUFA ethylester by glycerolysis under vacuum. Journal of Molecular catalysis B: Enzymatic, 4(4), 191-198(1998).

4. Akira Maehara, Kaoru Ikai, Shunsaku Ueda, and Tsuneo Yamane: Gene dosing effect on polyhydroxyalkanoates synthesis from n -alcohols in Paracoccus denitrificans . Biotechnology and Bioengineering, 60(1), 61-69(1998). [PubMed]

5. Shoji Ohuchi, Hideo Nakano and Tsuneo Yamane: In vitro method for the generation of protein libraries using PCR amplification of a single DNA molecule and coupled transcription/translation. Nucleic Acids Research, 26(19), 4339-4346 (1998). [PubMed]

6. Roxana Rosu, Yugo Iwasaki, Nobuyoshi Shimizu, Nobushige Doisaki, and Tsuneo Yamane: Intensification of lipase performance in a transesterification reaction by immobilization on CaCO 3 powder. Journal of Biotechnology, 66(1), 51-60(1998).

（２）総説(Reviews)

1. Hideo Nakano and Tsuneo Yamane: Cell-free protein synthesis systems. Biotechnology Advances, 16(2), 367-384(1998).

2. 山根恒夫：酵素の脂質分析への応用.日本油化学会誌、47(10), 1005-1013(1998).

3. Tsuneo Yamane, Yugo Iwasaki, Rosu Roxana, Nobuyoshi Shimizu, and Nobushige Doisaki: Multiple Intensified Performance of an Enzyme-Catalyzed Reaction in Organic Medium.Enzyme Engineering XIV [The Annals of the New York Academy ofSciences, 864, 171-179(1998)] [PubMed]

4. 山根恒夫、中野秀雄、河原崎泰昌：無細胞たん白質合成. ケミカル・エンジニアリング、43(3) , 198-206(1998).

（３）その他の刊行物 (Other Articles)

1. 山根恒夫：酵素の利用。I.　生物機能の利用。１．化学工学の進歩３２、「生体工学」（化学工学会編）槇書店(1998).

 

 1997

（１）原著報文(Original Papers)

1. Rosu, R., Uozaki, Y., Iwasaki, Y. and Yamane, T.: Repeated use of immobilized lipase for monoacylglycerol production by solid phase glycerolysis of olive oil. Journal of the American Oil Chemists' Society, 74 (4), 445-450(1997).

2. Nakano, H., Matsuda, K., Okumura, R. and Yamane, T.: Accumulation of translational inhibitors during multi-hour cell-free protein synthesis reaction using rabbit reticulocyte lysate. Journal of Fermentation and Bioengineering , 83 (5), 470-473(1997).

3. Miura, Y. and Yamane, T.: Epoxidation of alkenes and cycloalkenes by microbial lipases immobilized with photo-crosslinkable resin prepolymer. Biotechnology Letters , 19 (7), 611-613(1997).

4. Miura, T. and Yamane, T.: Screening for fungi that have high lipolytic and acidolytic activities in biomass support particles. Bioscience, Biotechnology and Biochemistry , 61 (8), 1252-1257(1997).

5. Mishima, N., Mizumoto, K., Iwasaki, Y., Nakano, H. and Yamane, T.: Insertion of stabilizing loci in vectors of T7 RNA polymerase-mediated Escherichia coli expression systems: A case study on the plasmids involving foreign phospholipase D gene. Biotechnology Progress , 13 (6), 864-868(1997). [PubMed]

6. Yoshida, Y., Kawase, M., Yamaguchi, C. and Yamane, T.: Enzymatic synthesis of estolides by a bioreactor. Journal of the American Oil Chemists' Society, 74 (3), 261-267 (1997)

その他の刊行物

1. 山根恒夫 : リン脂質の酵素工学.　化学工業、48(5), 396-403(1997).

2. 山根恒夫、塩谷捨明(編著) : バイオプロセスの知的制御. 序論、および計測技術の最近の進歩(第１章). 共立出版(株) (1997).

3. 山根恒夫 : バイオリアクター. 生物工学基礎コース、第８巻、｢バイオケミカル･エンジニアリング｣(佐田栄三、砂本順三編)、第２章、丸善(株)(1997).

 

 1996

（１）原著報文(Original Papers)

1. Ueda, S., Yabutani, T., Maehara, A. and Yamane, T.: Molecular analysis of the poly(3-hydroxyalkanoate) Synthesis gene from a methylotrophic bacterium, Paracoccus denitrificans . Journal of Baceriology, 178(2),774-779(1996). [PubMed]

2. Yamane, T., Chen, X.-F. and Ueda, S.: Growth associated production of poly(3-hydroxyvalerate) from n-pentanol by a methylotrophic bacterium, Paracoccus denitrificans . Applied and Environmental Microbiology, 62(2), 380-384(1996).

3. Yamane, T., Chen, X.-F. and Ueda, S.: Effect of the concentrations of alcohols on the compo-sition of polyhydroxyalcanoates produced during the growth of Paracoccus denitrificans . FEMS Microbiology Letters, 135(1), 207-211(1996).

4. Yamane, T., Chen, X.-F. and Ueda, S.: Polyhydroxyalkanoate synthesis from alcohols during the growth of Paracoccus denitrificans . FEMS Microbiology Letters, 135:207-211 (1996).

5. Yamane, T., Fukunaga, M. and Lee, Y.-W.: Increased PHB productivity by high-cell-density fed-batch culture of Alcaligenes latus , a growth-associated PHB producer. Biotechnology and Bioengineering, 50:197-202 (1996).

6. Shinonaga, M., Kawamura, Y., Shimbo, K. and Yamane, T.: Continuous Production of phospholipase D by Streptomyces Iydicus D-121 Immobilized with cross-linked chitosan beads. Journal of Fermentation and Bioengineering, 81:310-314 (1996).

7. Nakano, H., Tanaka, T., Kawarasaki, Y. and Yamane, T.: Highly productive cell-free protein synthesis system using condensed wheat-germ extract. Journal of Biotechnology, 46:275-282 (1996).

8. Kawarasaki, Y., Nakano, H. and Yamane, T.: Purification and some properties of wheat germ acid phosphatases. Plant Science, 119:67-77 (1996).

（２）総説(Reviews)

1. Nakano, H., Kawarasaki, Y. and Yamane, T.: Recent Advances in Cell-free Protein Synthesis towards a Protein Biosynthesizer. Enzyme Engineering XIII (The Annals of the New York Academy of Sciences), 799:406-412 (1996).

（３）その他の刊行物 (Other Articles)

1. 山根恒夫：油脂加工とバイオリアクター．日本食品科学工学会誌、43:463-471 (1996).

2. 山根恒夫：廃プラスチックの回収再利用および生分解性プラスチックの開発．「食品産業における廃棄物からの有価物分離・利用技術」、p.35-52,「食品産業エコ・プロセス技術研究組合」編集、(1996).